李瑶瑶,司娟宁,吴思进

• 1:北京信息科技大学仪器科学与光电工程学院

摘要(Abstract)：

为研究电针刺激下大脑血流实时响应特性，应用激光散斑衬比成像技术研究了电针刺激对大鼠脑皮层血流的影响。首先，设计了一款低成本、便携式激光散斑血流成像仪，通过闭塞后反应性充血实验验证了仪器的稳定性和可靠性。然后，基于此仪器进行肌肉电刺激实验，研究不同参数的电针刺激对大鼠脑皮层血流灌注的影响。研究表明，肌肉电刺激可以改变大鼠脑皮层血流灌注水平，较高的电刺激强度对脑血流的促进作用更强，较高的电刺激频率对脑血流的抑制作用更强。

关键词(KeyWords)： 激光散斑衬比成像;电针刺激;脑皮层血流

Abstract:

Keywords:

基金项目(Foundation): 北京市自然科学基金项目(7232049);; 国家自然科学基金项目(52075045);; “一带一路”国际科学组织联盟联合研究合作专项子课题(ANSO-CR-KP-2022-10)

作者(Author): 李瑶瑶,司娟宁,吴思进

DOI: 10.16508/j.cnki.11-5866/n.2024.06.010

参考文献(References)：

• [1] 张锦德，檀邹，林黎升，等.激光散斑成像在血流监测中的研究进展[J].中国激光医学杂志，2016,25(5):233-241.ZHANG J D,TAN Z,LIN L S,et al.Recent advances in monitoring blood flow with laser speckle imaging[J].Chinese Journal of Laser Medicine & Surgery,2016,25(5):233-241.(in Chinese)
• [2] 孔平，杨晖，郑刚，等.激光散斑血流成像技术研究新进展[J].光学技术，2014,40(1):21-26.KONG P,YANG H,ZHENG G,et al.Advances in laser speckle flowgraphy technique[J].Optical Technique,2014,40(1):21-26.(in Chinese)
• [3] 陈斌，陶静，黄佳，等.从Notch通路探讨电针促进局灶性脑缺血再灌注大鼠海马神经干细胞增殖的作用机制[J].中国康复医学杂志，2014,29(5):399-404.CHEN B,TAO J,HUANG J,et al.The possible mechanism of electroacupuncture promoting hippocampal neural stem cells proliferation in rats with focal cerebral ischemia-reperfusion via activation of Notch signaling pathway[J].Chinese Journal of Rehabilitation Medicine,2014,29(5):399-404.(in Chinese)
• [4] 欧阳钢，贾少微，王凡，等.不同电针刺激频率对脑卒中患者脑血流灌注和脑功能的影响[J].中国针灸，2005,25(11):776-778.OUYANG G,JIA S W,WANG F,et al.Effects of electroacupuncture of different frequencies on cerebral blood perfusion and cerebral function in the patient of stroke[J].Chinese Acupuncture & Moxibustion,2005,25(11):776-778.(in Chinese)
• [5] 张昉，孙智晶.神经肌肉电刺激预防深静脉血栓研究进展[J].现代妇产科进展，2017,26(8):634-636.ZHANG F,SUN Z J.Research progress on neuromuscular electrical stimulation for preventing deep vein thrombosis[J].Progress in Obstetrics and Gynecology,2017,26(8):634-636.(in Chinese)
• [6] 吴茂厚，詹荔琼，韩琼，等.神经肌肉电刺激联合血流限制训练在前交叉韧带重建术后早期康复中的应用疗效[J].中外医学研究，2021,19(22):140-143.WU M H,ZHAN L Q,HAN Q,et al.Effect of neuromuscular electrical stimulation combined with blood flow restriction training in early rehabilitation after anterior cruciate ligament reconstruction[J].Chinese and Foreign Medical Research,2021,19(22):140-143.(in Chinese)
• [7] BALKE M,TESCHLER M,SCH?FER H,et al.Therapeutic potential of electromyostimulation (EMS) in critically ill patients:a systematic review[J].Frontiers in Physiology,2022,13:865437.
• [8] JIN H K,HWANG T Y,CHO S H.Effect of electrical stimulation on blood flow velocity and vessel size[J].Open Medicine,2017,12(1):5-11.
• [9] SENARATHNA J,ABHISHEK R,NAN L,et al.Laser speckle contrast imaging theory,instrument[J].IEEE Reviews in Biomedical Engineering,2013,6:99-110.
• [10] BRIERS D,DUNCAN D D,HIRST E,et al.Laser speckle contrast imaging:theoretical and practical limitations[J].Journal of Biomedical Optics,2013,18(6):066018.
• [11] 夏萍，陈柳青.585 nm脉冲染料激光治疗鲜红斑痣前后激光散斑对比成像观察分析[J].中国中西医结合皮肤性病学杂志，2022,21(6):490-493.XIA P,CHEN L Q.Investigation of laser speckle contrast imaging before and after 585 nm pulsed dye laser treatment of port wine stains[J].Chinese Journal of Dermatovenerology of Integrated Traditional and Western Medicine,2022,21(6):490-493.(in Chinese)
• [12] 傅雪年，吴思进，司娟宁，等.激光散斑衬比成像在脑血流检测中的应用[J].科技创新与应用，2022,12(29):61-64.FU X N,WU S J,SI J N,et al.Application of laser speckle contrast imaging in cerebral blood flow detection [J].Technology Innovation and Application,2022,12(29):61-64.(in Chinese)
• [13] NIKOLAOS K,EUGENIA G,ANTONI-OS L,et al.Skin microvascular dysfunction in systemic lupus erythematosus patients with and without cardiovascular risk factors[J].Rheumatology,2021,60(6):2834-2841.
• [14] YIN L,YU T F,CHENG L G,et al.Laser speckle contrast imaging for blood flow monitoring in predicting outcomes after cerebral ischemia-reperfusion injury in mice[J].BMC Neuroscience,2022,23(1):80.
• [15] 许光，禹婧祎，刘书朋，等.基于激光散斑成像的外科手术术中无标记实时血流监测方法[J].工业控制计算机，2020,33(6):48-49.XU G,YU J Y,LIU S P,et al.Intraoperative label-free blood flow measurement based on laser speckle imaging[J].Industrial Control Computer,2020,33(6):48-49.(in Chinese)
• [16] BANDYOPADHYAY R,GITTINGS A S,SUH S S,et al.Speckle-visibility spectroscopy:a tool to study time-varying dynamics[J].The Review of Scientific Instruments,2005,76(9):093110.
• [17] FU X N,WU S J,SI J N,et al.A fusion denoising method based on homomorphic transform and 3D transform-domain collaborative filtering for laser speckle imaging of blood flow[J].Journal of Modern Optics,2022,69(19):1103-1114.
• [18] 唐元梁，李桂香，姚立平，等.皮肤微血管调节功能无创检测技术进展[J].中国医疗设备，2022,37(6):153-157.TANG Y L,LI G X,YAO L P,et al.Advances in noninvasive detection techniques of skin microvascular regulation function[J].China Medical Devices,2022,37(6):153-157.(in Chinese)
• [19] JAKOVELS D,RUBINS U,SPIGULIS J.LASCA and PPG imaging for non-contact assessment of skin blood supply[C]// Progress in Biomedical Optics and Imaging:Proceedings of SPIE.Lake Buena Vista,FL,US:SPIE,2013,8668:866849.
• [20] HORN A G,SCHULZE K M,WEBER R E,et al.Post-occlusive reactive hyperemia and skeletal muscle capillary hemodynamics[J].Microvascular Research,2022,140:104283.
• [21] ANDO S,TAKAGI Y,WATANABE H,et al.Effects of electrical muscle stimulation on cerebral blood flow[J].BMC Neuroscience,2021,22(1):67.
• [22] ZHOU F,GUO J C,CHENG J S,et al.Electroacupuncture increased cerebral blood flow and reduced ischemic brain injury:dependence on stimulation intensity and frequency[J].Journal of Applied Physiology,2011,111(6):1877-1887.
• [23] 张京，吴海艳，肖娟.吞咽肌不同强度电刺激对急性缺血性卒中吞咽障碍患者脑血流灌注参数的影响[J].中国全科医学，2016,19(2):164-169.ZHANG J,WU H Y,XIAO J.Influence of different intensities of electric stimulation to swallowing muscle on the parameters of cerebral blood flow of acute ischemic stroke patients with dysphagia[J].Chinese General Practice,2016,19(2):164-169.(in Chinese)
• [24] 范秀玉，毛忠南，张晓凌，等.电针基于不同参数治疗脑卒中后吞咽障碍的研究进展[J].中医药信息，2023,40(6):76-81.FAN X Y,MAO Z N,ZHANG X L,et al.Research progress of EA in treatment of post-stroke dysphagia based on different parameters[J].Information on Traditional Chinese Medicine,2023,40(6):76-81.(in Chinese)